Purpose: Impaired mucociliary clearance is an initial characteristic of recurrent cough, respiratory infection and chronic respiratory diseases. It has been demonstrated that prolonged inhalation of respirable silica particles results in a variety of pulmonary diseases, but whether the mucociliary system is involved in this process is unclear. This study aims to evaluate the effects of silica particles on mucociliary structure and MUC5B production in respiratory tract.
Materials and Methods: C57BL/6 mice were administered with 2.5 mg silica particles through a single intratracheal instillation. The changes of mucociliary structure and MUC5B expression in trachea was evaluated by HE and AB-PAS staining, transmission electronmicroscopy and immunohistochemistry on days 1, 7, 28 and 84 post-exposure.
Results: The mucociliary structure of airway epithelium was obviously impaired by silica particles, showing disordered, shortened or partially lost cilia on the surface, increased mucus in mucous layer and submucosal glands from day 7 to day 84. A variety of ultrastructural abnormalities were discovered in silica-exposed airway cilia, including absence of central pair microtubules, disorganized microtubules and clusters of axoneme on day 1 and 7.
The numbers of ciliary axonemes and basal bodies in ciliated epithelial cells were significantly decreased, whereas the proportion of abnormal axonemes was gradually increased with exposure to silica particles (P < 0.05). In addition, silica particles significantly decreased MUC5B expression on the surface of airway epithelium on day 28 and 84, but obviously increased its production in submucosal glands from day 1 to day 84 (P < 0.01).
DNA Gel Extraction Kit - 250 Columns |
31030-250 |
Biotium |
1kit |
EUR 545 |
Description: Minimum order quantity: 1 unit of 1kit |
Mouse Tail Genomic DNA Extraction Kit(250) |
M9100-250 |
GenDepot |
250 prep |
EUR 536 |
NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls |
80215-250 |
Alpha Diagnostics |
1 kit |
EUR 164 |
AXYPREP MAG DYECLEAN KIT- 250 ML - 25000 PREPS. |
MAG-DYECL-250 |
CORNING |
1/pk |
EUR 4182 |
Description: Bioscience Mag Beads; Magnetic Dye |
Disposable Meat Sample Plastic tubes with caps (5 ml) (250/pk) |
MST5-250 |
Alpha Diagnostics |
1 pk |
EUR 103 |
250 ML SPINNER FLASK, 70MM+B707 CENTRE NECK, 2 X 32 MM SIDEARMS |
4500-250 |
CORNING |
1/pk |
EUR 502 |
Description: Bioprocess Vessels; Spinner Flask 70 |
AXYPREP FRAGMENTSELECT-I KIT- 250 ML - 1900 PREPS |
MAG-FRAG-I-250 |
CORNING |
1/pk |
EUR 1953 |
Description: Bioscience Mag Beads; Magnetic Fragment Select |
AXYPREP MAG PCR NORMALIZER KIT- 250 ML - 1920 PREPS |
MAG-PCR-NM-250 |
CORNING |
1/pk |
EUR 1835 |
Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
AKAP 250 Polyclonal Antibody |
ES8070-100ul |
ELK Biotech |
100ul |
EUR 279 |
Description: A Rabbit Polyclonal antibody against AKAP 250 from Human. This antibody is tested and validated for IHC, IF, WB, ELISA |
AKAP 250 Polyclonal Antibody |
ES8070-50ul |
ELK Biotech |
50ul |
EUR 207 |
Description: A Rabbit Polyclonal antibody against AKAP 250 from Human. This antibody is tested and validated for IHC, IF, WB, ELISA |
Green Lysis Kit 250 |
GREENE5 |
Next Advance |
250pack |
EUR 468 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. |
Orange Lysis Kit 250 |
ORANGEE5-RNA |
Next Advance |
250pack |
EUR 537 |
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
Pink Lysis Kit 250 |
PINKE5 |
Next Advance |
250pack |
EUR 457 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. |
Navy Lysis Kit 250 |
NAVYE5 |
Next Advance |
250pack |
EUR 547 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. |
PLASTIC CLAMP, 250 ML |
480150 |
CORNING |
1/pk |
EUR 65 |
Description: Lab Equipment; Constant Temperature Equipment |
AKAP 250 Polyclonal Antibody |
ABP57071-003ml |
Abbkine |
0.03ml |
EUR 158 |
|
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350 |
AKAP 250 Polyclonal Antibody |
ABP57071-01ml |
Abbkine |
0.1ml |
EUR 289 |
|
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350 |
AKAP 250 Polyclonal Antibody |
ABP57071-02ml |
Abbkine |
0.2ml |
EUR 414 |
|
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350 |
Anti-AKAP 250 antibody |
STJ91531 |
St John's Laboratory |
200 µl |
EUR 197 |
Description: Rabbit polyclonal to AKAP 250. |
Red Lysis Kit 250 |
REDE5 |
Next Advance |
250pack |
EUR 520 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. |
YELLOW Lysis Kit 250 |
YELLOWE5 |
Next Advance |
250pack |
EUR 548 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. |
TGFBR2 (pS225 / 250) Antibody |
abx333097-100ul |
Abbexa |
100 ul |
EUR 467 |
|
PEG3-tetrazine, 250 mg |
1978-250mg |
Lumiprobe |
250 mg |
EUR 305 |
Gold Colloid (250 nm) |
62R-GC016 |
Fitzgerald |
20 ml |
EUR 154 |
Description: 250 nm Colloidal Gold particle suspension |
TGFBR2 antibody (Ser225/250) |
70R-36861 |
Fitzgerald |
100 ug |
EUR 327 |
Description: Rabbit Polyclonal TGFBR2 antibody (Ser225/250) |
pepsine cleaning solution 250 ml |
B231 |
Consort |
ea |
EUR 45 |
5.0ml Tip, Natural, 250/Pack |
BT999 |
Bio Basic |
1PK, 250UNIT |
EUR 74.88 |
|
Green RNA Lysis Kit 250 |
GREENE5-RNA |
Next Advance |
250pack |
EUR 571 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free. |
Green RINO Lysis Kit 250 |
GREENR5 |
Next Advance |
250pack |
EUR 483 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. |
Orange RINO Lysis Kit 250 |
ORANGER5-RNA |
Next Advance |
250pack |
EUR 554 |
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free. |
Pink RNA Lysis Kit 250 |
PINKE5-RNA |
Next Advance |
250pack |
EUR 561 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
Pink RINO Lysis Kit 250 |
PINKR5 |
Next Advance |
250pack |
EUR 473 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. |
Navy RINO Lysis Kit 250 |
NAVYR5 |
Next Advance |
250pack |
EUR 563 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. |
Navy RNA Lysis Kit 250 |
NAVYE5-RNA |
Next Advance |
250pack |
EUR 651 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free. |
Coomassie brilliant blue R-250 |
CB0037 |
Bio Basic |
5g |
EUR 58.7 |
|
Coomassie brilliant blue G-250 |
CB0038 |
Bio Basic |
25g |
EUR 75.23 |
|
250 ML DMSO (DIMETHYL SULFOXIDE) |
25-950-CQC |
CORNING |
250 mL/pk |
EUR 94 |
Description: Media Catalog; Cell Culture Reagents |
Coomassie brilliant blue R-250 |
20-abx082400 |
Abbexa |
|
|
|
Coomassie brilliant blue G-250 |
20-abx082402 |
Abbexa |
|
|
|
Coomassie brilliant blue R-250 |
20-abx082561 |
Abbexa |
|
|
|
Coomassie brilliant blue G-250 |
20-abx082562 |
Abbexa |
|
|
|
Centrosomal Protein 250 (CNAP1) Antibody |
abx231792-100ug |
Abbexa |
100 ug |
EUR 481 |
|
SharpProtein MarkerII unit: 250 µl |
FYP005-250UL |
Yeastern Biotech |
250ul |
Ask for price |
dUTP 100 mM 250 µl |
BT10601 |
Bioatlas |
250µl |
EUR 80 |
Description: High purity buffer for various PCR applications. |
Polyclonal STEAP4 Antibody (aa201-250) |
APR10293G |
Leading Biology |
0.05ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human STEAP4 (aa201-250). This antibody is tested and proven to work in the following applications: |
sox2 (Phospho-Ser249/250) Antibody |
13291-100ul |
SAB |
100ul |
EUR 252 |
sox2 (Phospho-Ser249/250) Antibody |
13291-50ul |
SAB |
50ul |
EUR 187 |
Red RNA Lysis Kit 250 |
REDE5-RNA |
Next Advance |
250pack |
EUR 622 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
Red RINO Lysis Kit 250 |
REDR5 |
Next Advance |
250pack |
EUR 534 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. |
YELLOW RNA Lysis Kit 250 |
YELLOWE5-RNA |
Next Advance |
250pack |
EUR 624 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free. |
YELLOW RINO Lysis Kit 250 |
YELLOWR5 |
Next Advance |
250pack |
EUR 562 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. |
Polyclonal CYP1B1 Antibody (aa200-250) |
APG02856G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP1B1 (aa200-250). This antibody is tested and proven to work in the following applications: |
Polyclonal DAB1 Antibody (aa201-250) |
APG03240G |
Leading Biology |
0.05ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DAB1 (aa201-250). This antibody is tested and proven to work in the following applications: |
Phospho-TGFBR2 (Ser225/250) Antibody |
CSB-PA911102- |
Cusabio |
|
EUR 335 |
|
Description: A polyclonal antibody against Phospho-TGFBR2 (Ser225/250). Recognizes Phospho-TGFBR2 (Ser225/250) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
Phospho-TGFBR2 (Ser225/250) Antibody |
CSB-PA911102-100ul |
Cusabio |
100ul |
EUR 362 |
|
Description: A polyclonal antibody against Phospho-TGFBR2 (Ser225/250). Recognizes Phospho-TGFBR2 (Ser225/250) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
IL-1a (223-250) (human) |
H-8290.0500 |
Bachem |
0.5mg |
EUR 708 |
Description: Sum Formula: C158H219N37O44; CAS# [130915-89-8] |
IL-1a (223-250) (human) |
H-8290.1000 |
Bachem |
1.0mg |
EUR 1240 |
Description: Sum Formula: C158H219N37O44; CAS# [130915-89-8] |
Coomassie Brilliant Blue R-250 |
CH024 |
ABM |
10 g |
EUR 121 |
Coomassie Brilliant Blue R-250 |
CH025 |
ABM |
25 g |
EUR 145 |
Coomassie Brilliant Blue R-250 |
CH026 |
ABM |
50 g |
EUR 175 |
Phospho-sox2 (Ser249/250) Antibody |
AF7450 |
Affbiotech |
200ul |
EUR 376 |
Description: Phospho-sox2 (Ser249/250) Antibody detects endogenous levels of sox2 only when phosphorylated at Ser249/250. |
JWH-250, Synthetic Cannabinoid Antibody |
6206-200 |
Biovision |
|
EUR 338 |
Sulfo-Cyanine5 dUTP, 250 nmol |
1510-250nmol |
Lumiprobe |
250 nmol |
EUR 735 |
Sulfo-Cyanine3 dUTP, 250 nmol |
1511-250nmol |
Lumiprobe |
250 nmol |
EUR 735 |
Sulfo-Cyanine5.5 dUTP, 250 nmol |
2071-250nmol |
Lumiprobe |
250 nmol |
EUR 735 |
Alkyne Amidite, hydroxyprolinol, 250 mg |
21360 |
Lumiprobe |
250 mg |
EUR 97 |
Pyrene phosphoramidite dU, 250 mg |
21460 |
Lumiprobe |
250 mg |
EUR 245 |
Biotin phosphoramidite (hydroxyprolinol), 250 mg |
22360 |
Lumiprobe |
250 mg |
EUR 197 |
Chemical phosphorylation amidite, 250 mg |
22560 |
Lumiprobe |
250 mg |
EUR 193 |
Long trebler phosphoramidite, 250 mg |
24260 |
Lumiprobe |
250 mg |
EUR 149 |
Alkyne CPG 1000A, 250 mg |
25860 |
Lumiprobe |
250 mg |
EUR 115 |
Hexaethylene glycol phosphoramidite, 250 mg |
26160 |
Lumiprobe |
250 mg |
EUR 82 |
Centrosomal Protein 250 kDa (CEP250) Antibody |
20-abx149235 |
Abbexa |
|
|
|
Green RINO RNA Lysis Kit 250 |
GREENR5-RNA |
Next Advance |
250pack |
EUR 587 |
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free. |
Pink RINO RNA Lysis Kit 250 |
PINKR5-RNA |
Next Advance |
250pack |
EUR 576 |
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
Navy RINO RNA Lysis Kit 250 |
NAVYR5-RNA |
Next Advance |
250pack |
EUR 666 |
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free. |
Centrosomal Protein 250 kDa (CEP250) Antibody |
20-abx111547 |
Abbexa |
|
|
|
BRG1-Associated Factor 250 (BAF250) Antibody |
20-abx007322 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Centrosomal Protein 250 kDa (CEP250) Antibody |
abx231601-100ug |
Abbexa |
100 ug |
EUR 481 |
|
Polyclonal TAAR1 / TA1 Antibody (aa225-250) |
APR13668G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TAAR1 / TA1 (aa225-250). This antibody is tested and proven to work in the following applications: |
Polyclonal STRADB / ALS2CR2 Antibody (aa200-250) |
APR10310G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human STRADB / ALS2CR2 (aa200-250). This antibody is tested and proven to work in the following applications: |
Polyclonal JWH-250, Synthetic Cannabinoid Antibody |
APR16964G |
Leading Biology |
0.1mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JWH-250, Synthetic Cannabinoid . This antibody is tested and proven to work in the following applications: |
Polyclonal BAF53 / ACTL6A Antibody (aa201-250) |
APR15124G |
Leading Biology |
0.05ml |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAF53 / ACTL6A (aa201-250). This antibody is tested and proven to work in the following applications: |
Polyclonal PTAR2 / FNTA Antibody (aa150-250) |
APR02433G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PTAR2 / FNTA (aa150-250). This antibody is tested and proven to work in the following applications: |
Polyclonal Latexin / MUM Antibody (aa200-250) |
APR02973G |
Leading Biology |
0.05mg |
EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Latexin / MUM (aa200-250). This antibody is tested and proven to work in the following applications: |
Red RINO RNA Lysis Kit 250 |
REDR5-RNA |
Next Advance |
250pack |
EUR 638 |
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
YELLOW RINO RNA Lysis Kit 250 |
YELLOWR5-RNA |
Next Advance |
250pack |
EUR 656 |
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free. |
Centrosomal Protein 250 kDa (CEP250) Antibody |
20-abx333700 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
sox2 (Phospho-Ser249/250) Conjugated Antibody |
C13291 |
SAB |
100ul |
EUR 397 |
Conclusions: Silica particles could lead to ultrastructural defects in airway cilia, mucus hypersecretion and altered MUC5B expression in trachea, indicating that impaired mucociliary structure and altered MUC5B production might participate in the development of silica-related respiratory diseases.