Sinq Systems

Purpose: Impaired mucociliary clearance is an initial characteristic of recurrent cough, respiratory infection and chronic respiratory diseases. It has been demonstrated that prolonged inhalation of respirable silica particles results in a variety of pulmonary diseases, but whether the mucociliary system is involved in this process is unclear. This study aims to evaluate the effects of silica particles on mucociliary structure and MUC5B production in respiratory tract.

Materials and Methods: C57BL/6 mice were administered with 2.5 mg silica particles through a single intratracheal instillation. The changes of mucociliary structure and MUC5B expression in trachea was evaluated by HE and AB-PAS staining, transmission electronmicroscopy and immunohistochemistry on days 1, 7, 28 and 84 post-exposure.

Results: The mucociliary structure of airway epithelium was obviously impaired by silica particles, showing disordered, shortened or partially lost cilia on the surface, increased mucus in mucous layer and submucosal glands from day 7 to day 84. A variety of ultrastructural abnormalities were discovered in silica-exposed airway cilia, including absence of central pair microtubules, disorganized microtubules and clusters of axoneme on day 1 and 7.

The numbers of ciliary axonemes and basal bodies in ciliated epithelial cells were significantly decreased, whereas the proportion of abnormal axonemes was gradually increased with exposure to silica particles (P < 0.05). In addition, silica particles significantly decreased MUC5B expression on the surface of airway epithelium on day 28 and 84, but obviously increased its production in submucosal glands from day 1 to day 84 (P < 0.01).

DNA Gel Extraction Kit - 250 Columns
31030-250	Biotium	1kit	EUR 545
Description: Minimum order quantity: 1 unit of 1kit

Mouse Tail Genomic DNA Extraction Kit(250)
M9100-250	GenDepot	250 prep	EUR 536

NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls
80215-250	Alpha Diagnostics	1 kit	EUR 164

AXYPREP MAG DYECLEAN KIT- 250 ML - 25000 PREPS.
MAG-DYECL-250	CORNING	1/pk	EUR 4182
Description: Bioscience Mag Beads; Magnetic Dye

Disposable Meat Sample Plastic tubes with caps (5 ml) (250/pk)
MST5-250	Alpha Diagnostics	1 pk	EUR 103

250 ML SPINNER FLASK, 70MM+B707 CENTRE NECK, 2 X 32 MM SIDEARMS
4500-250	CORNING	1/pk	EUR 502
Description: Bioprocess Vessels; Spinner Flask 70

AXYPREP FRAGMENTSELECT-I KIT- 250 ML - 1900 PREPS
MAG-FRAG-I-250	CORNING	1/pk	EUR 1953
Description: Bioscience Mag Beads; Magnetic Fragment Select

AXYPREP MAG PCR NORMALIZER KIT- 250 ML - 1920 PREPS
MAG-PCR-NM-250	CORNING	1/pk	EUR 1835
Description: Bioscience Mag Beads; Magnetic PCR Normaliser

Trypsin 1:250
TB0627	Bio Basic	50g	EUR 84.8

AKAP 250 Polyclonal Antibody
ES8070-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against AKAP 250 from Human. This antibody is tested and validated for IHC, IF, WB, ELISA

AKAP 250 Polyclonal Antibody
ES8070-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against AKAP 250 from Human. This antibody is tested and validated for IHC, IF, WB, ELISA

Green Lysis Kit 250
GREENE5	Next Advance	250pack	EUR 468
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads.

Orange Lysis Kit 250
ORANGEE5-RNA	Next Advance	250pack	EUR 537
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink Lysis Kit 250
PINKE5	Next Advance	250pack	EUR 457
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads.

Navy Lysis Kit 250
NAVYE5	Next Advance	250pack	EUR 547
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads.

PLASTIC CLAMP, 250 ML
480150	CORNING	1/pk	EUR 65
Description: Lab Equipment; Constant Temperature Equipment

AKAP 250 Polyclonal Antibody
ABP57071-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350

AKAP 250 Polyclonal Antibody
ABP57071-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350

AKAP 250 Polyclonal Antibody
ABP57071-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of AKAP 250 from Human. This AKAP 250 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AKAP 250 at AA range: 270-350

Anti-AKAP 250 antibody
STJ91531	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to AKAP 250.

ScrF I unit: 250
YRSCRF1	Yeastern Biotech	1 vial	Ask for price

Red Lysis Kit 250
REDE5	Next Advance	250pack	EUR 520
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads.

YELLOW Lysis Kit 250
YELLOWE5	Next Advance	250pack	EUR 548
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads.

TGFBR2 (pS225 / 250) Antibody
abx333097-100ul	Abbexa	100 ul	EUR 467

Peroxidase (250 U/mg)
HRP-02	Alpha Diagnostics	100 mg	EUR 712

PEG3-tetrazine, 250 mg
1978-250mg	Lumiprobe	250 mg	EUR 305

Gold Colloid (250 nm)
62R-GC016	Fitzgerald	20 ml	EUR 154
Description: 250 nm Colloidal Gold particle suspension

TGFBR2 antibody (Ser225/250)
70R-36861	Fitzgerald	100 ug	EUR 327
Description: Rabbit Polyclonal TGFBR2 antibody (Ser225/250)

pepsine cleaning solution 250 ml
B231	Consort	ea	EUR 45

5.0ml Tip, Natural, 250/Pack
BT999	Bio Basic	1PK, 250UNIT	EUR 74.88

Green RNA Lysis Kit 250
GREENE5-RNA	Next Advance	250pack	EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO Lysis Kit 250
GREENR5	Next Advance	250pack	EUR 483
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads.

Orange RINO Lysis Kit 250
ORANGER5-RNA	Next Advance	250pack	EUR 554
Description: Bead lysis kits for extracting bacteria or generating single cell suspensions from tissue samples. Pack of 250, 1.5mL RINO tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA	Next Advance	250pack	EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO Lysis Kit 250
PINKR5	Next Advance	250pack	EUR 473
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads.

Navy RINO Lysis Kit 250
NAVYR5	Next Advance	250pack	EUR 563
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads.

Navy RNA Lysis Kit 250
NAVYE5-RNA	Next Advance	250pack	EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Coomassie brilliant blue R-250
CB0037	Bio Basic	5g	EUR 58.7

Coomassie brilliant blue G-250
CB0038	Bio Basic	25g	EUR 75.23

250 ML DMSO (DIMETHYL SULFOXIDE)
25-950-CQC	CORNING	250 mL/pk	EUR 94
Description: Media Catalog; Cell Culture Reagents

Coomassie brilliant blue R-250
20-abx082400	Abbexa	EUR 230.00 EUR 189.00	25 g 5 g

Coomassie brilliant blue G-250
20-abx082402	Abbexa	EUR 230.00 EUR 189.00	25 g 5 g

Coomassie brilliant blue R-250
20-abx082561	Abbexa	EUR 217.00 EUR 175.00	25 g 5 g

Coomassie brilliant blue G-250
20-abx082562	Abbexa	EUR 217.00 EUR 175.00	25 g 5 g

IL-1a (223-250) (human)
5-01373	CHI Scientific	4 x 1mg	Ask for price

Centrosomal Protein 250 (CNAP1) Antibody
abx231792-100ug	Abbexa	100 ug	EUR 481

SharpProtein MarkerII unit: 250 µl
FYP005-250UL	Yeastern Biotech	250ul	Ask for price

dUTP 100 mM 250 µl
BT10601	Bioatlas	250µl	EUR 80
Description: High purity buffer for various PCR applications.

Polyclonal STEAP4 Antibody (aa201-250)
APR10293G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human STEAP4 (aa201-250). This antibody is tested and proven to work in the following applications:

sox2 (Phospho-Ser249/250) Antibody
13291-100ul	SAB	100ul	EUR 252

sox2 (Phospho-Ser249/250) Antibody
13291-50ul	SAB	50ul	EUR 187

Red RNA Lysis Kit 250
REDE5-RNA	Next Advance	250pack	EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO Lysis Kit 250
REDR5	Next Advance	250pack	EUR 534
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA	Next Advance	250pack	EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO Lysis Kit 250
YELLOWR5	Next Advance	250pack	EUR 562
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads.

Polyclonal CYP1B1 Antibody (aa200-250)
APG02856G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP1B1 (aa200-250). This antibody is tested and proven to work in the following applications:

Polyclonal DAB1 Antibody (aa201-250)
APG03240G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DAB1 (aa201-250). This antibody is tested and proven to work in the following applications:

10G Coomassie Blue R-250
NAT1360	National Diagnostics	10G	EUR 103

10G Coomassie Blue G-250
NAT1362	National Diagnostics	10G	EUR 148

Phospho-TGFBR2 (Ser225/250) Antibody
CSB-PA911102-	Cusabio		EUR 335
Description: A polyclonal antibody against Phospho-TGFBR2 (Ser225/250). Recognizes Phospho-TGFBR2 (Ser225/250) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

Phospho-TGFBR2 (Ser225/250) Antibody
CSB-PA911102-100ul	Cusabio	100ul	EUR 362
Description: A polyclonal antibody against Phospho-TGFBR2 (Ser225/250). Recognizes Phospho-TGFBR2 (Ser225/250) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100

IL-1a (223-250) (human)
H-8290.0500	Bachem	0.5mg	EUR 708
Description: Sum Formula: C158H219N37O44; CAS# [130915-89-8]

IL-1a (223-250) (human)
H-8290.1000	Bachem	1.0mg	EUR 1240
Description: Sum Formula: C158H219N37O44; CAS# [130915-89-8]

Coomassie Brilliant Blue R-250
CH024	ABM	10 g	EUR 121

Coomassie Brilliant Blue R-250
CH025	ABM	25 g	EUR 145

Coomassie Brilliant Blue R-250
CH026	ABM	50 g	EUR 175

Phospho-sox2 (Ser249/250) Antibody
AF7450	Affbiotech	200ul	EUR 376
Description: Phospho-sox2 (Ser249/250) Antibody detects endogenous levels of sox2 only when phosphorylated at Ser249/250.

JWH-250, Synthetic Cannabinoid Antibody
6206-200	Biovision		EUR 338

Sulfo-Cyanine5 dUTP, 250 nmol
1510-250nmol	Lumiprobe	250 nmol	EUR 735

Sulfo-Cyanine3 dUTP, 250 nmol
1511-250nmol	Lumiprobe	250 nmol	EUR 735

Sulfo-Cyanine5.5 dUTP, 250 nmol
2071-250nmol	Lumiprobe	250 nmol	EUR 735

Alkyne Amidite, hydroxyprolinol, 250 mg
21360	Lumiprobe	250 mg	EUR 97

Pyrene phosphoramidite dU, 250 mg
21460	Lumiprobe	250 mg	EUR 245

Biotin phosphoramidite (hydroxyprolinol), 250 mg
22360	Lumiprobe	250 mg	EUR 197

Chemical phosphorylation amidite, 250 mg
22560	Lumiprobe	250 mg	EUR 193

Long trebler phosphoramidite, 250 mg
24260	Lumiprobe	250 mg	EUR 149

Alkyne CPG 1000A, 250 mg
25860	Lumiprobe	250 mg	EUR 115

Hexaethylene glycol phosphoramidite, 250 mg
26160	Lumiprobe	250 mg	EUR 82

Centrosomal Protein 250 kDa (CEP250) Antibody
20-abx149235	Abbexa	EUR 425.00 EUR 342.00	100 ug 50 ug

Green RINO RNA Lysis Kit 250
GREENR5-RNA	Next Advance	250pack	EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA	Next Advance	250pack	EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA	Next Advance	250pack	EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Centrosomal Protein 250 kDa (CEP250) Antibody
20-abx111547	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

BRG1-Associated Factor 250 (BAF250) Antibody
20-abx007322	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Centrosomal Protein 250 kDa (CEP250) Antibody
abx231601-100ug	Abbexa	100 ug	EUR 481

Polyclonal TAAR1 / TA1 Antibody (aa225-250)
APR13668G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TAAR1 / TA1 (aa225-250). This antibody is tested and proven to work in the following applications:

Polyclonal STRADB / ALS2CR2 Antibody (aa200-250)
APR10310G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human STRADB / ALS2CR2 (aa200-250). This antibody is tested and proven to work in the following applications:

Polyclonal JWH-250, Synthetic Cannabinoid Antibody
APR16964G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human JWH-250, Synthetic Cannabinoid . This antibody is tested and proven to work in the following applications:

Polyclonal BAF53 / ACTL6A Antibody (aa201-250)
APR15124G	Leading Biology	0.05ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAF53 / ACTL6A (aa201-250). This antibody is tested and proven to work in the following applications:

Polyclonal PTAR2 / FNTA Antibody (aa150-250)
APR02433G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PTAR2 / FNTA (aa150-250). This antibody is tested and proven to work in the following applications:

Polyclonal Latexin / MUM Antibody (aa200-250)
APR02973G	Leading Biology	0.05mg	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Latexin / MUM (aa200-250). This antibody is tested and proven to work in the following applications:

Red RINO RNA Lysis Kit 250
REDR5-RNA	Next Advance	250pack	EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA	Next Advance	250pack	EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Centrosomal Protein 250 kDa (CEP250) Antibody
20-abx333700	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

T-Pro Plasmid Mini Kit (250)
RB94-YPD250	T-Pro Biotechnology	250preps/Kit	EUR 222

sox2 (Phospho-Ser249/250) Conjugated Antibody
C13291	SAB	100ul	EUR 397

Conclusions: Silica particles could lead to ultrastructural defects in airway cilia, mucus hypersecretion and altered MUC5B expression in trachea, indicating that impaired mucociliary structure and altered MUC5B production might participate in the development of silica-related respiratory diseases.