Purpose: Impaired mucociliary clearance is an initial characteristic of recurrent cough, respiratory infection and chronic respiratory diseases. It has been demonstrated that prolonged inhalation of respirable silica particles results in a variety of pulmonary diseases, but whether the mucociliary system is involved in this process is unclear. This study aims to evaluate the effects of silica particles on mucociliary structure and MUC5B production in respiratory tract.

Materials and Methods: C57BL/6 mice were administered with 2.5 mg silica particles through a single intratracheal instillation. The changes of mucociliary structure and MUC5B expression in trachea was evaluated by HE and AB-PAS staining, transmission electronmicroscopy and immunohistochemistry on days 1, 7, 28 and 84 post-exposure.

Results: The mucociliary structure of airway epithelium was obviously impaired by silica particles, showing disordered, shortened or partially lost cilia on the surface, increased mucus in mucous layer and submucosal glands from day 7 to day 84. A variety of ultrastructural abnormalities were discovered in silica-exposed airway cilia, including absence of central pair microtubules, disorganized microtubules and clusters of axoneme on day 1 and 7.

The numbers of ciliary axonemes and basal bodies in ciliated epithelial cells were significantly decreased, whereas the proportion of abnormal axonemes was gradually increased with exposure to silica particles (P < 0.05). In addition, silica particles significantly decreased MUC5B expression on the surface of airway epithelium on day 28 and 84, but obviously increased its production in submucosal glands from day 1 to day 84 (P < 0.01).

SU C18 5um 250 x 4.6mm - EACH
CHR4392 EACH
EUR 977.4

SY C18 5um 250 x 4.6mm - EACH
CHR4498 EACH
EUR 1026

LU C18 10um 250 x 4.6mm - EACH
CHR3946 EACH
EUR 876.15

uB C18 10um 250 x 4.6mm - EACH
CHR4560 EACH
EUR 700.65

C18 Polar 5um 250 x 4.6mm - EACH
CHR4878 EACH
EUR 904.5

C18 AB-D C18 5um 250 x 4.6mm - EACH
CHR4854 EACH
EUR 904.5

LU C18(2) 5um 250 x 4.6mm - EACH
CHR3978 EACH
EUR 920.7

AT T3 C18 5um 250 x 4.6mm - EACH
CHR4618 EACH
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HY BDS C18 5um 250 x 4.6mm - EACH
CHR3728 EACH
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LU C18(2) 10um 250 x 4.6mm - EACH
CHR3962 EACH
EUR 652.05

HY Gold C18 5um 250 x 4.6mm - EACH
CHR3714 EACH
EUR 880.2

Carboxymethyl Cellulose Sodium Salt (CMC Sodium), Medium Viscosity 250-350CPS
93797 500 Gms
EUR 5.13
Description: Part A

Absolute Mag™ C18 Silica Magnetic Particles, 250 nm
SMP-NM06 10 mL
EUR 814

cd36 250 250 nm 2 ml
cd36-250 2 ML
EUR 367.5

cd38 250 250 nm 2 ml
cd38-250 2 ML
EUR 519.75

JWH 250
J211500 10mg
EUR 58
Description: 864445-43-2

JWH 250
MBS6069661-10mg 10(mg
EUR 505

JWH 250
MBS6069661-5x10mg 5x10mg
EUR 2125

NIK-250
MBS5777866-5mg 5(mg
EUR 915

NIK-250
MBS5777866-5x5mg 5x5(mg
EUR 3970

NIK-250
T28173-10mg 10mg Ask for price
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PIC 383-250*250-B7541 AC Signs
227699 Card of 1 Pictogram(s)
EUR 5.99
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PIC 384-250*250-B7541 AC Signs
227700 Card of 1 Pictogram(s)
EUR 5.99
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BI 376-250*250-B0455 AC Signs
227703 Card of 1 Pictogram(s)
EUR 27.66
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V 376-250*250-B0455 AC Signs
227707 Card of 1 Pictogram(s)
EUR 31.14
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PIC 377-250*250-B7541 AC Signs
223636 Card of 1 Pictogram(s)
EUR 5.99
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PIC 372-250*250-B7541 AC Signs
223994 Card of 1 Pictogram(s)
EUR 5.99
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PIC 352-250*250-B7541 AC Signs
224141 Card of 1 Pictogram(s)
EUR 5.99
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PIC 374-250*250-B7541 AC Signs
224143 Card of 1 Pictogram(s)
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PIC 353-250*250-B7541 AC Signs
224145 Card of 1 Pictogram(s)
EUR 5.99
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PIC 354-250*250-B7541 AC Signs
224147 Card of 1 Pictogram(s)
EUR 5.99
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PIC 355-250*250-B7541 AC Signs
224149 Card of 1 Pictogram(s)
EUR 5.99
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PIC 356-250*250-B7541 AC Signs
224151 Card of 1 Pictogram(s)
EUR 5.99
Description: Description Dutch: Reddingspictogram - Veiligheidsdouche; Description French: Panneau d'évacuation - Douche de sécurié

PIC 350-250*250-B7541 AC Signs
224153 Card of 1 Pictogram(s)
EUR 5.99
Description: Description Dutch: Reddingspictogram - Te volgen richting; Description French: Panneau d'évacuation - Flèche directionnelle

PIC 351-250*250-B7541 AC Signs
224155 Card of 1 Pictogram(s)
EUR 5.99
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PIC 372-250*250-B7541 AC Signs
224157 Card of 1 Pictogram(s)
EUR 5.99
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PIC 382-250*250-B7541 AC Signs
224160 Card of 1 Pictogram(s)
EUR 5.99
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PIC 376-250*250-B7541 AC Signs
224166 Card of 1 Pictogram(s)
EUR 5.99
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PIC 375-250*250-B7541 AC Signs
224168 Card of 1 Pictogram(s)
EUR 5.99
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PIC 391-250*250-B7541 AC Signs
224170 Card of 1 Pictogram(s)
EUR 5.99
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PIC 390-250*250-B7541 AC Signs
224172 Card of 1 Pictogram(s)
EUR 5.99
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PIC 391-250*250-B7527 AC Signs
252324 Card of 1 Pictogram(s)
EUR 11.64
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PIC 350-250*250-B7527 AC Signs
252401 Card of 1 Pictogram(s)
EUR 11.64
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PIC 351-250*250-B7527 AC Signs
252403 Card of 1 Pictogram(s)
EUR 11.64
Description: Description Dutch: Reddingspictogram - Pijl; Description French: Panneau d'évacuation - Flèche directionnelle

PIC 353-250*250-B7527 AC Signs
252407 Card of 1 Pictogram(s)
EUR 11.64
Description: Description Dutch: Reddingspictogram - Arts; Description French: Panneau d'évacuation - Médecin

PIC 354-250*250-B7527 AC Signs
252409 Card of 1 Pictogram(s)
EUR 11.64
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PIC 356-250*250-B7527 AC Signs
252413 Card of 1 Pictogram(s)
EUR 11.64
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PIC 372-250*250-B7527 AC Signs
252445 Card of 1 Pictogram(s)
EUR 11.64
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PIC 374-250*250-B7527 AC Signs
252459 Card of 1 Pictogram(s)
EUR 11.64
Description: Description Dutch: Reddingspictogram - Telefoon voor redding en eerste hulp; Description French: Panneau d'évacuation - Téléphone de secours

PIC 375-250*250-B7527 AC Signs
252461 Card of 1 Pictogram(s)
EUR 11.64
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BI 372-250*250-B0455 AC Signs
253507 Card of 1 Pictogram(s)
EUR 27.66
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BI 352-250*250-B0455 AC Signs
253509 Card of 1 Pictogram(s)
EUR 27.66
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BI 354-250*250-B0455 AC Signs
253511 Card of 1 Pictogram(s)
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253513 Card of 1 Pictogram(s)
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BI 403-250*250-B0455 AC Signs
253532 Card of 1 Pictogram(s)
EUR 27.66
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V 403-250*250-B0455 AC Signs
253533 Card of 1 Pictogram(s)
EUR 31.14
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BI 401-250*250-B0455 AC Signs
253534 Card of 1 Pictogram(s)
EUR 27.66
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V 401-250*250-B0455 AC Signs
253535 Card of 1 Pictogram(s)
EUR 31.14
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BI 412-250*250-B0455 AC Signs
253536 Card of 1 Pictogram(s)
EUR 27.66
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V 412-250*250-B0455 AC Signs
253537 Card of 1 Pictogram(s)
EUR 31.14
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BI 402-250*250-B0455 AC Signs
253548 Card of 1 Pictogram(s)
EUR 27.66
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253551 Card of 1 Pictogram(s)
EUR 31.14
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V 372-250*250-B0455 AC Signs
253607 Card of 1 Pictogram(s)
EUR 31.14
Description: Description Dutch: Wigvormige reddingspictogrammen - Uitgang; Description French: Panneau d'évacuation - Sortie

V 352-250*250-B0455 AC Signs
253609 Card of 1 Pictogram(s)
EUR 31.14
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V 354-250*250-B0455 AC Signs
253611 Card of 1 Pictogram(s)
EUR 31.14
Description: Description Dutch: Wigvormige reddingspictogrammen - Ogen spoelen; Description French: Panneau d'évacuation - Rinçage des yeux

V 356-250*250-B0455 AC Signs
253613 Card of 1 Pictogram(s)
EUR 31.14
Description: Description Dutch: Wigvormige reddingspictogrammen - Veiligheidsdouche; Description French: Panneau d'évacuation - Douche de sécurié

PIC 241-250*250-B7527 AC Signs
255346 Card of 1 Pictogram(s)
EUR 11.64
Description: Description Dutch: Informatietekens - Uitgang; Description French: Sortie

Caprisil C18-CB HPLC Column, 3 µm, 100 Å, CE533-C18-CB x 250 mm
CE433-C18-CB 3 µm Ask for price
Description: Caprisil C18-CB

Caprisil C18-CB HPLC Column, 5 µm, 100 Å, CE553-C18-CB x 250 mm
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Description: Caprisil C18-CB

Caprisil C18-CB HPLC Column, 3 µm, 100 Å, CE533-C18-CB x 250 mm
CE533-C18-CB 3 µm Ask for price
Description: Caprisil C18-CB

Caprisil C18-CB HPLC Column, 5 µm, 100 Å, CE553-C18-CB x 250 mm
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Caprisil C18 HPLC Column, 3 µm, 100 Å, CE533-C18 x 250 mm
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Caprisil C30 HPLC Column, 3 µm, 100 Å, CE533-C30 x 250 mm
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Caprisil C18 HPLC Column, 3 µm, 300 Å, CE538-C18 x 250 mm
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Caprisil C18 HPLC Column, 5 µm, 100 Å, CE553-C18 x 250 mm
CE453-C18 5 µm Ask for price
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Caprisil C30 HPLC Column, 5 µm, 100 Å, CE553-C30 x 250 mm
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Caprisil C18 HPLC Column, 5 µm, 300 Å, CE558-C18 x 250 mm
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Caprisil C18 HPLC Column, 3 µm, 100 Å, CE533-C18 x 250 mm
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Caprisil C30 HPLC Column, 3 µm, 100 Å, CE533-C30 x 250 mm
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Caprisil C18 HPLC Column, 3 µm, 300 Å, CE538-C18 x 250 mm
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Caprisil C18 HPLC Column, 5 µm, 100 Å, CE553-C18 x 250 mm
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Caprisil C30 HPLC Column, 5 µm, 100 Å, CE553-C30 x 250 mm
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Caprisil C18 HPLC Column, 5 µm, 300 Å, CE558-C18 x 250 mm
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Description: Caprisil C18

BPE, 250 mg
0414 250 mg
EUR 348.8

Trypsin 1:250
35537-52 25G
EUR 80.5

tag-250 Antibody
CSB-PA339444XA01CXY-02mg 0.2mg Ask for price
Description: Recombinant Caenorhabditis elegans tag-250 protein

tag-250 Antibody
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Description: Recombinant Caenorhabditis elegans tag-250 protein

CTGF (182-250)
cyt-526 4µg
EUR 60
Description: Recombinant Human Connective Tissue Growth Factor (182-250 a.a.)

AKAP 250 Antibody
C10861-100ul 100μl
EUR 217
Description: AKAP 250 Rabbit Polyclonal Antibody

AKAP 250 Antibody
C10861-50ul 50μl
EUR 143.5
Description: AKAP 250 Rabbit Polyclonal Antibody

JWH 250-d11
J211502 10mg
EUR 242

JWH 250-d11
MBS6018940-1mg 1(mg
EUR 605

JWH 250-d11
MBS6018940-5x1mg 5x1mg
EUR 2565

250 uL Syringe
MLCP-250muSYR-1 250 uL
EUR 73
Description: 250 uL Syringe

Trypsin 1:250
TB0627 50g
EUR 101.76

CTGF (182-250)
cyt-526-01 4µg Ask for price

CTGF (182-250)
cyt-526-02 20µg Ask for price

CTGF (182-250)
cyt-526-03 1mg Ask for price

JWH 250-d11
TRC-J211502-10MG 10 mg Ask for price

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Timing Belt 250
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Description: Cuvette Tray

Trypsin 1:250
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Caprisil C18-B HPLC Column, 3 µm, 100 Å, CE533-C18-B x 250 mm
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Caprisil C18-P HPLC Column, 3 µm, 100 Å, CE553-C18-P x 250 mm
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Caprisil C18-X HPLC Column, 3 µm, 100 Å, CE535-C18-X x 250 mm
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Caprisil C18-B HPLC Column, 3 µm, 300 Å, CE538-C18-B x 250 mm
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Caprisil C18-B HPLC Column, 5 µm, 100 Å, CE553-C18-B x 250 mm
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Caprisil C18-P HPLC Column, 5 µm, 100 Å, CE553-C18-P x 250 mm
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Caprisil C18-X HPLC Column, 5 µm, 100 Å, CE555-C18-X x 250 mm
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Caprisil C18-B HPLC Column, 5 µm, 300 Å, CE558-C18-B x 250 mm
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Caprisil C18-B HPLC Column, 3 µm, 100 Å, CE533-C18-B x 250 mm
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Caprisil C18-X HPLC Column, 3 µm, 100 Å, CE535-C18-X x 250 mm
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Caprisil C18-B HPLC Column, 3 µm, 300 Å, CE538-C18-B x 250 mm
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Caprisil C18-B HPLC Column, 5 µm, 100 Å, CE553-C18-B x 250 mm
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Caprisil C18-P HPLC Column, 3 µm, 100 Å, CE553-C18-P x 250 mm
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Description: Caprisil C18-P

Caprisil C18-X HPLC Column, 5 µm, 100 Å, CE555-C18-X x 250 mm
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Caprisil C18-B HPLC Column, 5 µm, 300 Å, CE558-C18-B x 250 mm
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Description: Caprisil C18-B

MUSEUM JARS RECTANGLR 250 X 250 X 120 MM
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Caprisil C18-AQ HPLC Column, 3 µm, 100 Å, CE533-C18-AQ x 250 mm
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Description: Caprisil C18-AQ

Caprisil C18-TH HPLC Column, 3 µm, 100 Å, CE533-C18-TH x 250 mm
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Caprisil C18-IP HPLC Column, 3 µm, 100 Å, CE535-C18-IP x 250 mm
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Caprisil C18-LC HPLC Column, 3 µm, 100 Å, CE535-C18-LC x 250 mm
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Caprisil C18-XS HPLC Column, 3 µm, 100 Å, CE535-C18-XS x 250 mm
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Caprisil C18-AQ HPLC Column, 5 µm, 100 Å, CE553-C18-AQ x 250 mm
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Caprisil C18-TH HPLC Column, 5 µm, 100 Å, CE553-C18-TH x 250 mm
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Caprisil C18-IP HPLC Column, 5 µm, 100 Å, CE555-C18-IP x 250 mm
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Caprisil C18-LC HPLC Column, 5 µm, 100 Å, CE555-C18-LC x 250 mm
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Caprisil C18-XS HPLC Column, 5 µm, 100 Å, CE555-C18-XS x 250 mm
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Caprisil C18-AQ HPLC Column, 3 µm, 100 Å, CE533-C18-AQ x 250 mm
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Description: Caprisil C18-AQ

Caprisil C18-TH HPLC Column, 3 µm, 100 Å, CE533-C18-TH x 250 mm
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Description: Caprisil C18-TH

Caprisil C18-IP HPLC Column, 3 µm, 100 Å, CE535-C18-IP x 250 mm
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Description: Caprisil C18-IP

Caprisil C18-LC HPLC Column, 3 µm, 100 Å, CE535-C18-LC x 250 mm
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Caprisil C18-XS HPLC Column, 3 µm, 100 Å, CE535-C18-XS x 250 mm
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Description: Caprisil C18-XS

Caprisil C18-AQ HPLC Column, 5 µm, 100 Å, CE553-C18-AQ x 250 mm
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Caprisil C18-TH HPLC Column, 5 µm, 100 Å, CE553-C18-TH x 250 mm
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Description: Caprisil C18-TH

Caprisil C18-IP HPLC Column, 5 µm, 100 Å, CE555-C18-IP x 250 mm
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Description: Caprisil C18-IP

Caprisil C18-LC HPLC Column, 5 µm, 100 Å, CE555-C18-LC x 250 mm
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Description: Caprisil C18-LC

Caprisil C18-XS HPLC Column, 5 µm, 100 Å, CE555-C18-XS x 250 mm
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Description: Caprisil C18-XS

Recombinant Caenorhabditis elegans Putative protein tag-250 (tag-250), partial
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Caprisil C18-AQ (2) HPLC Column, 3 µm, 100 Å, CE533-C18-AQ (2) x 250 mm
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Description: Caprisil C18-AQ (2)

Caprisil C18-AQ (2) HPLC Column, 5 µm, 100 Å, CE553-C18-AQ (2) x 250 mm
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Description: Caprisil C18-AQ (2)

Caprisil C18-AQ (2) HPLC Column, 3 µm, 100 Å, CE533-C18-AQ (2) x 250 mm
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Description: Caprisil C18-AQ (2)

Caprisil C18-AQ (2) HPLC Column, 5 µm, 100 Å, CE553-C18-AQ (2) x 250 mm
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Description: Caprisil C18-AQ (2)

CMKC-250 AB Labels
049125 Pack of 3600 Label(s)
EUR 139.42
Description: Description Dutch: Soldeermaskers voor printplaten; Description French: Masque de soudure pour circuits imprimés

Conclusions: Silica particles could lead to ultrastructural defects in airway cilia, mucus hypersecretion and altered MUC5B expression in trachea, indicating that impaired mucociliary structure and altered MUC5B production might participate in the development of silica-related respiratory diseases.