Sinq Systems

Mitochondrial damage is commonly involved in liver injury. We have previously shown that normal mitochondria can be coated with a carrier protein to form complexes that are specifically taken up by liver cells in culture.

The aim of the current study was to determine whether mitochondrial complexes could be specifically delivered to the livers of living rats by intravenous injection.Mitochondria were harvested from fresh mouse liver, mixed with an asialoglycoprotein-based carrier, asialoorosomucoid-polylysine (AsOR-PL), and purified to form complexes.

To facilitate release of internalized mitochondria from endosomes, an endosomolytic peptide, listeriolysin O (LLO) was coupled to AsOR to form AsOR-LLO. Mitochondria alone, mitochondrial complexes with AsOR-PL, and mitochondrial complexes plus AsOR-LLO conjugate all containing the same number of mitochondria were injected intravenously.

Animals were sacrificed, and organs removed and analyzed by quantitative polymerase chain reaction (qPCR) of mouse mitochondrial DNA, electronmicroscopy (EM), and in situ PCR and hybridization followed by immunohistochemical analyses.Calculations revealed that approximately 27% of the total injected mitochondria was detected in liver, while less than 2% was found in spleen, and <1% in lungs. 

Spastin(Sp 3G11-1), 1mg/mL
BNUM2844-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

FOXP3(3G3), 1mg/mL
BNUM0645-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Mass Set E2 1mg - 100g - EACH
BAL1746	Scientific Laboratory Supplies	EACH	EUR 2231.55

Mass Set E2 1mg - 200g - EACH
BAL1748	Scientific Laboratory Supplies	EACH	EUR 2693.25

Mass Set E2 1mg - 500g - EACH
BAL1750	Scientific Laboratory Supplies	EACH	EUR 2999.7

Mass Set Brass 1mg to 200g
BAL1606	Scientific Laboratory Supplies	ST	EUR 412.68

CD50 (ICAM-3)(186-2G9), 1mg/mL
BNUM0178-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Precision Balance 220g (1mg) - EACH
BAL1166	Scientific Laboratory Supplies	EACH	EUR 1404

Precision Balance 320g (1mg) - EACH
BAL1168	Scientific Laboratory Supplies	EACH	EUR 1559.25

Precision Balance 420g (1mg) - EACH
BAL1170	Scientific Laboratory Supplies	EACH	EUR 1707.75

Precision Balance 620g (1mg) - EACH
BAL1172	Scientific Laboratory Supplies	EACH	EUR 2004.75

Precision Balance 650g (1mg) - EACH
BAL1174	Scientific Laboratory Supplies	EACH	EUR 2301.75

Moisture Analyser - 120g . 1mg - EACH
MOI1002	Scientific Laboratory Supplies	EACH	EUR 6012.9

Glypican-3(GPC3/863 + 1G12), 1mg/mL
BNUM0864-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD29 (Stem Cell Marker)(12G10), 1mg/mL
BNUM2905-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

EGLN1 / PHD2(366G/76/3), 1mg/mL
BNUM3074-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Precision Balance int.cal. 220g (1mg) - EACH
BAL1002	Scientific Laboratory Supplies	EACH	EUR 1711.8

Precision Balance int.cal. 320g (1mg) - EACH
BAL1004	Scientific Laboratory Supplies	EACH	EUR 1846.8

Precision Balance int.cal. 420g (1mg) - EACH
BAL1006	Scientific Laboratory Supplies	EACH	EUR 1981.8

Precision Balance int.cal. 620g (1mg) - EACH
BAL1008	Scientific Laboratory Supplies	EACH	EUR 2266.65

Precision Balance int.cal. 650g (1mg) - EACH
BAL1024	Scientific Laboratory Supplies	EACH	EUR 2566.35

Mass Set E2 1mg - 100g Calibrated - EACH
BAL1762	Scientific Laboratory Supplies	EACH	EUR 2435.4

Mass Set E2 1mg - 200g Calibrated - EACH
BAL1764	Scientific Laboratory Supplies	EACH	EUR 2859.3

Mass Set E2 1mg - 500g Calibrated - EACH
BAL1766	Scientific Laboratory Supplies	EACH	EUR 3217.05

CDw17(H018.3G-6.F5), 1mg/mL
BNUM1092-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

TTF-1 / NKX2.1(8G7G3/1), 1mg/mL
BNUM0448-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

AX223/E Ext Cal 220g 1mg Balance - EACH
BAL0016	Scientific Laboratory Supplies	EACH	EUR 2045.25

AX423/E Ext Cal 420g 1mg Balance - EACH
BAL0018	Scientific Laboratory Supplies	EACH	EUR 2289.6

AX523/E Ext Cal 520g 1mg Balance - EACH
BAL0020	Scientific Laboratory Supplies	EACH	EUR 2817.45

Desmocollin-2/3 (7G6), 1mg/mL
BNUM1430-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Desmoglein-3 (Squamous Cell Marker) (5G11), 1mg/mL
BNUM1439-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Mass Set E2 1mg - 50g - EACH
BAL1744	Scientific Laboratory Supplies	EACH	EUR 2056.05

TTF-1 / NKX2.1(8G7G3/1 + NX2.1/690), 1mg/mL
BNUM0907-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

AX223 220g 1mg Int Cal Precision Balance - EACH
BAL0015	Scientific Laboratory Supplies	EACH	EUR 2470.5

AX423 420g 1mg Int Cal Precision Balance - EACH
BAL0017	Scientific Laboratory Supplies	EACH	EUR 2722.95

AX523 520g 1mg Int Cal Precision Balance - EACH
BAL0019	Scientific Laboratory Supplies	EACH	EUR 3244.05

Glucose Regulated Protein 94 (GRP94) (9G10.F8.2), 1mg/mL
BNUM0940-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Explorer EX223/E 220g x 1mg external cal - EACH
BAL5111	Scientific Laboratory Supplies	EACH	EUR 3518.1

Explorer EX423/E 420g x 1mg external cal - EACH
BAL5113	Scientific Laboratory Supplies	EACH	EUR 4442.85

Interferon gamma (IFNG)(G-23), 1mg/mL
BNUM0219-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Interferon gamma (IFNG)(G-30), 1mg/mL
BNUM0347-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Catenin, alpha-1 (CTNNA1) (1G5), 1mg/mL
BNUM1364-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Mass Set E2 1mg - 50g Calibrated - EACH
BAL1760	Scientific Laboratory Supplies	EACH	EUR 2177.55

OPCA03317-1MG - AFUA_4G09580 Recombinant Protein
OPCA03317-1MG	Aviva Systems Biology	1mg	EUR 1977

OPCA03335-1MG - AFUA_2G03830 Recombinant Protein
OPCA03335-1MG	Aviva Systems Biology	1mg	EUR 1977

Entris II Advance Preci Bal 220g Cap 1mg Read - EACH
BAL4576	Scientific Laboratory Supplies	EACH	EUR 3199.5

Entris II Advance Preci Bal 320g Cap 1mg Read - EACH
BAL4588	Scientific Laboratory Supplies	EACH	EUR 3451.95

Entris II Advance Preci Bal 420g Cap 1mg Read - EACH
BAL4598	Scientific Laboratory Supplies	EACH	EUR 3736.8

Entris II Advance Preci Bal 620g Cap 1mg Read - EACH
BAL4618	Scientific Laboratory Supplies	EACH	EUR 4336.2

CD44v3 (Marker of Tumor Metastasis)(3G5), 1mg/mL
BNUM2429-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Entris II Advance Preci Bal Int Cal 1200g Cap 1mg Read - EACH
BAL4558	Scientific Laboratory Supplies	EACH	EUR 6285.6

Anti-CD32 Antibody [2.4G2], Qfluor 630-1mg
QAB87-QF630-1mg	EnQuireBio	1mg	EUR 534

Accuris™ F1 grade calibration weight set, 23 pcs, 1mg to 200g
W1100-200	Benchmark Scientific	1 PC	EUR 542.6

Accuris™ M1 Grade Calibration Weight, Set, 21 pcs, 1mg to 100g
W1105-21-100	Benchmark Scientific	1 each	EUR 161.5

Accuris™ M1 Grade Calibration Weight, Set, 22 pcs, 1mg to 200g
W1105-22-200	Benchmark Scientific	1 each	EUR 193.85

Entris II Advance Preci Bal Int Cal 1200g Cap 1mg Read Veri - EACH
BAL4638	Scientific Laboratory Supplies	EACH	EUR 6901.2

Entris II Advance Preci Bal Int Cal 220g Cap 1mg Read - EACH
BAL4578	Scientific Laboratory Supplies	EACH	EUR 3531.6

Entris II Advance Preci Bal Int Cal 320g Cap 1mg Read - EACH
BAL4590	Scientific Laboratory Supplies	EACH	EUR 3832.65

Entris II Advance Preci Bal Int Cal 420g Cap 1mg Read - EACH
BAL4608	Scientific Laboratory Supplies	EACH	EUR 4118.85

Entris II Advance Preci Bal Int Cal 620g Cap 1mg Read - EACH
BAL4620	Scientific Laboratory Supplies	EACH	EUR 4677.75

Entris II Advance Preci Bal Int Cal 220g Cap 1mg Read Veri - EACH
BAL4648	Scientific Laboratory Supplies	EACH	EUR 4172.85

Entris II Advance Preci Bal Int Cal 320g Cap 1mg Read Veri - EACH
BAL4664	Scientific Laboratory Supplies	EACH	EUR 4395.6

Entris II Advance Preci Bal Int Cal 420g Cap 1mg Read Veri - EACH
BAL4670	Scientific Laboratory Supplies	EACH	EUR 4695.3

Entris II Advance Preci Bal Int Cal 620g Cap 1mg Read Veri - EACH
BAL4676	Scientific Laboratory Supplies	EACH	EUR 5282.55

Anti-CD32 Antibody [2.4G2], Unconjugated-1mg
QAB87-1mg	EnQuireBio	1mg	EUR 452.4

OPEF01669-1MG - Gastrin 17 (G-17) Antigen
OPEF01669-1MG	Aviva Systems Biology	1mg	EUR 949

OPEF01670-1MG - Gastrin 17 (G-17) Antigen
OPEF01670-1MG	Aviva Systems Biology	1mg	EUR 949

OAEF00738-1MG - Gastrin 17 (G-17) Antibody
OAEF00738-1MG	Aviva Systems Biology	1mg	EUR 1112

OAEF00779-1MG - Gastrin 17 (G-17) Antibody
OAEF00779-1MG	Aviva Systems Biology	1mg	EUR 1112

OAEF00780-1MG - Gastrin 17 (G-17) Antibody
OAEF00780-1MG	Aviva Systems Biology	1mg	EUR 1112

OAEF00781-1MG - Gastrin 17 (G-17) Antibody
OAEF00781-1MG	Aviva Systems Biology	1mg	EUR 1112

OPEF01671-1MG - Gastrin 17 (G-17) Antigen : BSA
OPEF01671-1MG	Aviva Systems Biology	1mg	EUR 949

BSA, 1mg/mL
22070005-1	Glycomatrix	25 mL	EUR 23.46

BSA, 1mg/mL
22070005-2	Glycomatrix	50 mL	EUR 39.2

BSA, 1mg/mL
22070005-3	Glycomatrix	1 mL	EUR 13.17

Collagen 1mg/ml
ABP-COL-T1	Abpcorp	3 x 0,5ml vials	EUR 97.2

Carrier RNA  1mg
CR000001	Geneaid Biotech	each	EUR 3

CD27(203.6), 1mg/mL
BNUM0163-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD28(204.12), 1mg/mL
BNUM0164-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD46(122.2), 1mg/mL
BNUM0175-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Recombinant Human Ubiquitin G76A Protein, Untagged, E.coli-1mg
QP13870-1mg	EnQuireBio	1mg	EUR 3427.2

Recombinant Streptavidin - 1mg
NRPA05S-1	HangZhou Neuro Peptide Biological Science and Technology Incorporation Limited(dotwaybio)	1mg	EUR 28.3

CD8(C8/1035), 1mg/mL
BNUM1035-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD25(143-13), 1mg/mL
BNUM0297-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD53(161-2), 1mg/mL
BNUM0324-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD26(202-36), 1mg/mL
BNUM0888-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD16(C16/1045), 1mg/mL
BNUM1045-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

pS2(TFF1/1091), 1mg/mL
BNUM1091-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD43(SPN/1094), 1mg/mL
BNUM1094-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD86(C86/1146), 1mg/mL
BNUM1146-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD28 (C28/1636), 1mg/mL
BNUM1636-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

ROR2(ROR2/1911), 1mg/mL
BNUM1911-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

ROR2(ROR2/1912), 1mg/mL
BNUM1912-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

MDM2(MDM2/2414), 1mg/mL
BNUM2414-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

NRF1(NRF1/2608), 1mg/mL
BNUM2608-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

NRF1(NRF1/2609), 1mg/mL
BNUM2609-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

MTF1(MTF1/2649), 1mg/mL
BNUM2649-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Tal1(TAL1/2707), 1mg/mL
BNUM2707-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Tal1(TAL1/3146), 1mg/mL
BNUM3146-50	Biotium	1uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

CD71(TFRC/1059), 1mg/mL
BNUM1059-50	Biotium	50uL	EUR 396
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application

Immunohistochemistry showed that mouse mitochondrial DNA staining was minimal with mitochondrial complexes alone, strong periportal with mitochondrial complexes co-injected with AsOR-LLO, and absent with mitochondria alone.Targetable mitochondrial complexes can be delivered to rat liver, and the efficiency of that process is greatly enhanced by co-injection of a targetable endosomal release agent, AsOR-LLO.