Targeted Delivery of Mitochondria to Liver in Rats.

Mitochondrial damage is commonly involved in liver injury. We have previously shown that normal mitochondria can be coated with a carrier protein to form complexes that are specifically taken up by liver cells in culture.

The aim of the current study was to determine whether mitochondrial complexes could be specifically delivered to the livers of living rats by intravenous injection.Mitochondria were harvested from fresh mouse liver, mixed with an asialoglycoprotein-based carrier, asialoorosomucoid-polylysine (AsOR-PL), and purified to form complexes.

To facilitate release of internalized mitochondria from endosomes, an endosomolytic peptide, listeriolysin O (LLO) was coupled to AsOR to form AsOR-LLO. Mitochondria alone, mitochondrial complexes with AsOR-PL, and mitochondrial complexes plus AsOR-LLO conjugate all containing the same number of mitochondria were injected intravenously.

Animals were sacrificed, and organs removed and analyzed by quantitative polymerase chain reaction (qPCR) of mouse mitochondrial DNA, electronmicroscopy (EM), and in situ PCR and hybridization followed by immunohistochemical analyses.Calculations revealed that approximately 27% of the total injected mitochondria was detected in liver, while less than 2% was found in spleen, and <1% in lungs. 

Recombinant Human FKBP6 Protein, His, -1mg

QP10610-1mg 1mg
EUR 5251

Anti-CD3e Antibody [145-2C11], Unconjugated-1mg

QAB2-1mg 1mg
EUR 251

Recombinant Human HK3 Protein, His, E.coli-1mg

QP12272-1mg 1mg
EUR 5251

Recombinant Human HMBS Protein, His, E.coli-1mg

QP12279-1mg 1mg
EUR 2757

Recombinant Human HMGA1 Protein, His, E.coli-1mg

QP12280-1mg 1mg
EUR 5251

Recombinant Human HMGB2 Protein, His, Baculovirus-1mg

QP12282-1mg 1mg
EUR 3655

Recombinant Human HMGB3 Protein, His, E.coli-1mg

QP12283-1mg 1mg
EUR 2757

Recombinant Human HMGN1 Protein, His, E.coli-1mg

QP12285-1mg 1mg
EUR 5251

Recombinant Human HMGN3 Protein, His, E.coli-1mg

QP12286-1mg 1mg
EUR 2757

Recombinant Human HMOX2 Protein, Untagged, E.coli-1mg

QP12287-1mg 1mg
EUR 2312

Recombinant Human HN1L Protein, His, E.coli-1mg

QP12288-1mg 1mg
EUR 2757

Recombinant Human HNMT Protein, His, E.coli-1mg

QP12289-1mg 1mg
EUR 3655

Recombinant Human HNRNPAB Protein, His, E.coli-1mg

QP12290-1mg 1mg
EUR 5251

Recombinant Human HOMER2 Protein, His, E.coli-1mg

QP12292-1mg 1mg
EUR 5251

Recombinant Human HOMER3 Protein, His, E.coli-1mg

QP12293-1mg 1mg
EUR 2757

Recombinant Human HOPX Protein, His, E.coli-1mg

QP12294-1mg 1mg
EUR 2757

Recombinant Human HOXA9 Protein, His, E.coli-1mg

QP12295-1mg 1mg
EUR 2757

Recombinant Human HOXB13 Protein, His, E.coli-1mg

QP12296-1mg 1mg
EUR 2757

Recombinant Human HOXC11 Protein, His, E.coli-1mg

QP12297-1mg 1mg
EUR 3655

Recombinant Human HPCAL1 Protein, His, E.coli-1mg

QP12298-1mg 1mg
EUR 5251

Recombinant Mouse HPGD Protein, His, E.coli-1mg

QP12300-1mg 1mg
EUR 2757

Recombinant Human HPGD Protein, His, E.coli-1mg

QP12301-1mg 1mg
EUR 5251

Recombinant Human HPGDS Protein, His, E.coli-1mg

QP12302-1mg 1mg
EUR 2757

Recombinant Human HPR Protein, His, E.coli-1mg

QP12303-1mg 1mg
EUR 5251

Recombinant Human HPSE Protein, His, E.coli-1mg

QP12304-1mg 1mg
EUR 2757

Recombinant Human HRAS Protein, His, E.coli-1mg

QP12311-1mg 1mg
EUR 2312

Recombinant Human HRASLS3 Protein, Untagged, E.coli-1mg

QP12312-1mg 1mg
EUR 1859

Recombinant Human HS3ST1 Protein, His, E.coli-1mg

QP12313-1mg 1mg
EUR 5251

Recombinant Human HSBP1L1 Protein, His, E.coli-1mg

QP12318-1mg 1mg
EUR 2757

Recombinant Human HSCB Protein, His, E.coli-1mg

QP12319-1mg 1mg
EUR 2757

Recombinant Human HSD17B1 Protein, His, E.coli-1mg

QP12320-1mg 1mg
EUR 5251

Recombinant Human HSD17B8 Protein, His, E.coli-1mg

QP12321-1mg 1mg
EUR 3655

Recombinant Human HSF1 Protein, His, E.coli-1mg

QP12322-1mg 1mg
EUR 3655

Recombinant Human HSF2BP Protein, His, E.coli-1mg

QP12323-1mg 1mg
EUR 2757

Recombinant Human HSFY1 Protein, His, E.coli-1mg

QP12324-1mg 1mg
EUR 2757

Recombinant Human HSP27 Protein, Untagged, E.coli-1mg

QP12327-1mg 1mg
EUR 1959

Recombinant Human HSPA1B Protein, His, E.coli-1mg

QP12328-1mg 1mg
EUR 5251

Recombinant Human HSPA8 Protein, His, E.coli-1mg

QP12329-1mg 1mg
EUR 5251

Recombinant Human HSPA9 Protein, His, E.coli-1mg

QP12330-1mg 1mg
EUR 1859

Recombinant Human HSPB11 Protein, His, E.coli-1mg

QP12331-1mg 1mg
EUR 2757

Recombinant Human HSPB3 Protein, His, E.coli-1mg

QP12332-1mg 1mg
EUR 2757

Recombinant Human HSPB6 Protein, His, E.coli-1mg

QP12333-1mg 1mg
EUR 5251

Recombinant Human HSPB7 Protein, His, E.coli-1mg

QP12334-1mg 1mg
EUR 5251

Recombinant Human HSPB9 Protein, His, E.coli-1mg

QP12336-1mg 1mg
EUR 5251

Recombinant Human HSPBP1 Protein, His, E.coli-1mg

QP12337-1mg 1mg
EUR 5251

Recombinant Human HSPH1 Protein, His, E.coli-1mg

QP12338-1mg 1mg
EUR 2757

Recombinant Human HTATIP2 Protein, His, E.coli-1mg

QP12350-1mg 1mg
EUR 2757

Recombinant Virus HTNV Protein, GST, E.coli-1mg

QP12359-1mg 1mg
EUR 1261

Recombinant Human HTRA2 Protein, His, E.coli-1mg

QP12360-1mg 1mg
EUR 1859

Recombinant Human HYKK Protein, His, E.coli-1mg

QP12362-1mg 1mg
EUR 5251

Recombinant Human ICAM2 Protein, His, E.coli-1mg

QP12363-1mg 1mg
EUR 5251

Recombinant Human ICOSLG Protein, His, Insect-1mg

QP12364-1mg 1mg
EUR 5251

Recombinant Human IDE Protein, His, E.coli-1mg

QP12365-1mg 1mg
EUR 5251

Recombinant Human IDI1 Protein, His, E.coli-1mg

QP12366-1mg 1mg
EUR 5251

Recombinant Human IDI2 Protein, His, E.coli-1mg

QP12367-1mg 1mg
EUR 3655

Recombinant E.coli IDNK Protein, His, E.coli-1mg

QP12368-1mg 1mg
EUR 2757

Recombinant Human IDS Protein, His, Insect-1mg

QP12369-1mg 1mg
EUR 5251

Recombinant Human IF Protein, His, Insect-1mg

QP12371-1mg 1mg
EUR 4453

Recombinant Human IFI30 Protein, His, E.coli-1mg

QP12372-1mg 1mg
EUR 5251

Recombinant Human IFNA7 Protein, His, E.coli-1mg

QP12375-1mg 1mg
EUR 5251

Recombinant Mouse IFNAR1 Protein, His, Insect-1mg

QP12376-1mg 1mg
EUR 5251

Recombinant Feline IFNG Protein, His, E.coli-1mg

QP12377-1mg 1mg
EUR 5251

Recombinant Canine IFNG Protein, His, E.coli-1mg

QP12378-1mg 1mg
EUR 2757

Recombinant Human IFT20 Protein, His, E.coli-1mg

QP12379-1mg 1mg
EUR 2757

Recombinant Mouse IGFBP6 Protein, His, Insect-1mg

QP12382-1mg 1mg
EUR 5251

Recombinant Human IGFBP7 Protein, Untagged, E.coli-1mg

QP12384-1mg 1mg
EUR 2757

Recombinant Human IGFLR1 Protein, His, E.coli-1mg

QP12385-1mg 1mg
EUR 5251

Recombinant Mouse IgG Protein, Untagged, Insect-1mg

QP12386-1mg 1mg
EUR 5251

Recombinant Human IGJ Protein, His, E.coli-1mg

QP12387-1mg 1mg
EUR 2757

Recombinant Human IGLL1 Protein, His, E.coli-1mg

QP12388-1mg 1mg
EUR 2757

Recombinant Human IL10RA Protein, His, E.coli-1mg

QP12401-1mg 1mg
EUR 5251

Recombinant Human IL10RB Protein, His, Insect-1mg

QP12402-1mg 1mg
EUR 5251

Recombinant Human IL13RA1 Protein, His, E.coli-1mg

QP12405-1mg 1mg
EUR 5251

Recombinant Human IL15RA Protein, His, Insect-1mg

QP12407-1mg 1mg
EUR 5251

Recombinant Human IL17RB Protein, His, Insect-1mg

QP12409-1mg 1mg
EUR 5251

Recombinant Mouse IL17RB Protein, His, Insect-1mg

QP12410-1mg 1mg
EUR 5251

Recombinant Human IL1R1 Protein, His, Insect-1mg

QP12412-1mg 1mg
EUR 5251

Recombinant Human IL21R Protein, His, Insect-1mg

QP12415-1mg 1mg
EUR 5251

Recombinant Human IL23R Protein, His, Insect-1mg

QP12416-1mg 1mg
EUR 5251

Recombinant Human IL28B Protein, His, Insect-1mg

QP12417-1mg 1mg
EUR 5251

Recombinant Human IL2RA Protein, His, Insect-1mg

QP12418-1mg 1mg
EUR 5251

Recombinant Human IL2RG Protein, His, Insect-1mg

QP12419-1mg 1mg
EUR 5251

Recombinant Canine IL31 Protein, His, Insect-1mg

QP12420-1mg 1mg
EUR 5251

Recombinant Human IL34 Protein, His, E.coli-1mg

QP12421-1mg 1mg
EUR 4734

Recombinant Mouse IL34 Protein, His, E.coli-1mg

QP12422-1mg 1mg
EUR 5251

Recombinant Mouse IL5RA Protein, His, Insect-1mg

QP12426-1mg 1mg
EUR 5251

Recombinant Canine IL6 Protein, His, Insect-1mg

QP12427-1mg 1mg
EUR 5251

Recombinant Mouse IL6R Protein, His, Insect-1mg

QP12428-1mg 1mg
EUR 5251

Recombinant Human IMMP2L Protein, His, E.coli-1mg

QP12430-1mg 1mg
EUR 2757

Recombinant Human IMP3 Protein, His, E.coli-1mg

QP12431-1mg 1mg
EUR 2757

Recombinant Human IMPA1 Protein, His, E.coli-1mg

QP12432-1mg 1mg
EUR 2757

Recombinant Human IMPA2 Protein, His, E.coli-1mg

QP12433-1mg 1mg
EUR 5251

Recombinant Human IMPACT Protein, His, E.coli-1mg

QP12434-1mg 1mg
EUR 2757

Recombinant Human IMPAD1 Protein, His, E.coli-1mg

QP12435-1mg 1mg
EUR 2757

Recombinant Mouse IMPAD1 Protein, His, Insect-1mg

QP12436-1mg 1mg
EUR 5251

Recombinant Human IMPDH1 Protein, His, E.coli-1mg

QP12437-1mg 1mg
EUR 5251

Recombinant Human ING1 Protein, His, E.coli-1mg

QP12445-1mg 1mg
EUR 2757

Recombinant Human ING2 Protein, His, E.coli-1mg

QP12446-1mg 1mg
EUR 2757

Recombinant Human IP6K2 Protein, His, E.coli-1mg

QP12454-1mg 1mg
EUR 5251

Recombinant Human ISG15 Protein, Untagged, E.coli-1mg

QP12460-1mg 1mg
EUR 1859

Immunohistochemistry showed that mouse mitochondrial DNA staining was minimal with mitochondrial complexes alone, strong periportal with mitochondrial complexes co-injected with AsOR-LLO, and absent with mitochondria alone.Targetable mitochondrial complexes can be delivered to rat liver, and the efficiency of that process is greatly enhanced by co-injection of a targetable endosomal release agent, AsOR-LLO.