Abstract
Wheat (Triticum aestivum L.), one of the most important crops, belongs to the Triticum genus of the Poaceae family. Some of the important cytoplasmic genes come from the chloroplast genome. In this study, the chloroplast genome of a pair of recombinant inbred lines of T. aestivum was sequenced, assembled, and annotated.
Our results show that this chloroplast genome consists of 137 unique genes, including 87 protein-coding genes, 42 tRNA genes, and 8 rRNA genes. A maximum-likelihood phylogenetic tree based on 15 chloroplast genomes revealed that the two T. aestivum are closely related to the genus Triticum. The chloroplast genome could be used for wheat species identification, cytoplasmic inheritance gene functional study, and breeding.
Keywords: Triticum aestivum Recombinants; chloroplast genome.
Qualification: analytical standard
Rehearsal: >95%
Molecular weight: 10kDa
Packaging: 250μg packet
Manufacturer/trade name: indoor biotechnologies
Technique(s)
- HPLC: suitable
- LC/MS: suitable
Applications: food and drinks
Format: organized
Storage temperature: −20°C
Related Categories
- Food and Cosmetic Component Standards
- Amino acid, peptide and protein standards
General description
Produced and qualified by Indoor Biotechnologies.
Source: E. coli
- Formulation: Without preservatives or carriers in 20 mM Tris, 0.3 M NaCl, 0.2 M L-arginine, pH 8.0. Filtered through a 0.22 µm filter.
- Concentration: See product insert.
- Purification: Purified from E. coli culture by multi-step HPLC chromatography. Purity >95% by SDS-PAGE
Note:
(1) rTri at 14 has a C-terminal 6xHis tag.
(2) Avoid repeated freeze/thaw cycles. It is recommended to aliquot the product on the first thaw and freeze in working aliquot volumes.
Disclaimer
The allergens are provided for in vitro research and commercial use: not for in vivo human or therapeutic use.