Volumetric imaging of samples utilizing fluorescence microscopy performs an vital position in varied fields together with bodily, medical and life sciences. Here we report a deep learning-based volumetric picture inference framework that makes use of 2D pictures which are sparsely captured by a typical wide-field fluorescence microscope at arbitrary axial positions throughout the pattern quantity. Through a recurrent convolutional neural community, which we time period as Recurrent-MZ, 2D fluorescence data from a couple of axial planes throughout the pattern is explicitly integrated to digitally reconstruct the pattern quantity over an prolonged depth-of-field. Using experiments on C. elegans and nanobead samples,
Recurrent-MZ is demonstrated to considerably improve the depth-of-field of a 63×/1.4NA goal lens, additionally offering a 30-fold discount within the variety of axial scans required to picture the identical pattern quantity. We additional illustrated the generalization of this recurrent community for 3D imaging by displaying its resilience to various imaging situations, together with e.g., totally different sequences of enter pictures, masking varied axial permutations and unknown axial positioning errors. We additionally demonstrated wide-field to confocal cross-modality picture transformations utilizing
Recurrent-MZ framework and carried out 3D picture reconstruction of a pattern utilizing a couple of wide-field 2D fluorescence pictures as enter, matching confocal microscopy pictures of the identical pattern quantity. Recurrent-MZ demonstrates the primary software of recurrent neural networks in microscopic picture reconstruction and gives a versatile and speedy volumetric imaging framework, overcoming the restrictions of present 3D scanning microscopy instruments. Palawan is the malaria-endemic province with the best prevalence of malaria within the Philippines, and microscopists (group well being staff) have been allowed to ship early analysis and immediate remedy of malaria all through the province since 1999.
To enhance the standard of care, the current mixed-methods research tried to determine the components related to satisfaction of sufferers in Palawan with their microscopists by analyzing the sufferers’ views. First, a quantitative cross-sectional research was performed amongst 48 ex-patients and their 9 microscopists. Ex-patients have been requested about their satisfaction with care, and microscopists have been requested about their job satisfaction and talent. Structural equation modeling was performed for information evaluation.
Super-resolving Microscopy in Neuroscience
Fluorescence imaging strategies play a pivotal position in our understanding of the nervous system. The emergence of varied super-resolution microscopy strategies and specialised fluorescent probes allows direct perception into neuronal construction and protein preparations in mobile subcompartments with up to now unmatched decision. Super-resolving visualization strategies in neurons unveil a novel understanding of cytoskeletal composition, distribution, motility, and signaling of membrane proteins, subsynaptic construction and performance, and neuron-glia interplay. Well-defined molecular targets in autoimmune and neurodegenerative.
Application of super-resolution microscopy in human mind samples and for testing scientific biomarkers remains to be in its infancy however opens new alternatives for translational analysis in neurology and neuroscience. In this assessment, we describe how super-resolving microscopy has improved our understanding of neuronal and mind perform and dysfunction within the final 20 years. Second, a qualitative cross-sectional research was carried out utilizing narrative interviews. Data have been analyzed utilizing the NVivo 10 software program program. As a end result, each research revealed excessive affected person satisfaction with microscopists.
The quantitative research confirmed that ex-patients’ satisfaction with their microscopists was independently enhanced by two components: excessive means in malaria microscopic commentary and low family wealth of the sufferers. Particularly, particular subpopulations (these with decrease family wealth, comparatively previous individuals, and ethnic minorities) have been extra glad with the microscopists’ care. The qualitative research strengthens this discovering by displaying that their microscopists provided immediate and exact analysis and efficient remedy at no cost. In conclusion, microscopists have been proven to have an vital position in narrowing the disparities in malaria care in Palawan.
Investigation of plant leaf-derived graphene quantum dot clusters through magnetic drive microscopy
Magnetic drive microscopy (MFM) is utilized to characterize the magnetic second in nanostructured plant leaf-derived graphene quantum dot clusters (GQDCs). The MFM sign reveals that the magnetic response of the GQDCs is dependent upon the peak and width of the GQDCs. However, particular person GQDs, and smaller clusters with widths of lower than 20 nm, haven’t proven any observable magnetic sign. Importantly, experimental analyses recommend that the magnetic sign of GQDCs distributed in a aircraft could be successfully detected at room temperature. These outcomes may pave the best way for future graphene-based magnetic storage media and spin manipulation quantum gadgets.
, light fraction) Paraffin oil (Mineral oil), light fraction |
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| PC5530 | Bio Basic | 100ml | EUR 58.7 |
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, heavy fraction) Paraffin oil (Mineral oil), heavy fraction |
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| PC5531 | Bio Basic | 100ml | EUR 58.7 |
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 Mineral oil |
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| abx082059-100ml | Abbexa | 100 ml | EUR 189 |
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Mineral oil |
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| abx082415-100ml | Abbexa | 100 ml | EUR 189 |
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 Immersion oil |
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| GW3483-100ML | Glentham Life Sciences | 100 ml | EUR 58 |
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Mineral oil |
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| GX1229-1L | Glentham Life Sciences | 1 l | EUR 48 |
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Mineral oil |
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| GX1229-250ML | Glentham Life Sciences | 250 ml | EUR 39 |
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Mineral oil |
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| GX1229-500ML | Glentham Life Sciences | 500 ml | EUR 43 |
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Mineral oil |
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 Clove oil |
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Clove oil |
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| GK9829-100ML | Glentham Life Sciences | 100 ml | EUR 54 |
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Peanut oil |
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| GK9829-1L | Glentham Life Sciences | 1 l | EUR 130 |
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Peanut oil |
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| GK9829-250ML | Glentham Life Sciences | 250 ml | EUR 70 |
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Peanut oil |
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| GK9829-500ML | Glentham Life Sciences | 500 ml | EUR 90 |
 Jojoba oil |
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| GL1962-100ML | Glentham Life Sciences | 100 ml | EUR 78 |
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Jojoba oil |
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Olive oil |
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| GL2537-100ML | Glentham Life Sciences | 100 ml | EUR 70 |
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Bergamot oil |
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| GL2537-1L | Glentham Life Sciences | 1 l | EUR 229 |
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Bergamot oil |
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Cottonseed oil |
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Soybean oil |
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Borage oil |
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| GL6882-100ML | Glentham Life Sciences | 100 ml | EUR 70 |
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Cedar oil |
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| GL6882-25ML | Glentham Life Sciences | 25 ml | EUR 46 |
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Cedar oil |
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| GL6882-500ML | Glentham Life Sciences | 500 ml | EUR 150 |
 Corn oil |
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| GL7753-500ML | Glentham Life Sciences | 500 ml | EUR 58 |
 Sesame oil |
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| GL9605-1L | Glentham Life Sciences | 1 l | EUR 102 |
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Sesame oil |
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| GL9605-250ML | Glentham Life Sciences | 250 ml | EUR 62 |
, extra heavy fraction) Paraffin oil (Mineral oil), extra heavy fraction |
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| PC5532 | Bio Basic | 100ml | EUR 58.7 |
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) Oil Red O Stain Kit (For Fat) |
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| ORK-1 | ScyTek Laboratories | 1 kit(s) | EUR 192 |
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Oil Red O Stain Kit (For Fat) |
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| ORK-2 | ScyTek Laboratories | 100 Slides | EUR 122 |
) DL-Methioninol (oil) |
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| 5-07713 | CHI Scientific | 25g | Ask for price |
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DL-Methioninol (oil) |
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 Valerian root oil |
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| 20-abx186597 | Abbexa |
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 Tea tree oil |
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| GK8936-100ML | Glentham Life Sciences | 100 ml | EUR 53 |
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Tea tree oil |
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| GK8936-1KG | Glentham Life Sciences | 1 kg | EUR 138 |
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| GK2233-100ML | Glentham Life Sciences | 100 ml | EUR 49 |
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Sunflower seed oil |
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 Oil Red O |
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| OD0395 | Bio Basic | 50g | EUR 65.66 |
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 Peanut Oil vaccine adjuvant |
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| AV-5010-50 | Alpha Diagnostics | 50 ml | EUR 225 |
 Mineral Oil vaccine adjuvant |
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| AV-5020-50 | Alpha Diagnostics | 50 ml | EUR 225 |
) Z-Leu-OH (oil) |
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| C-2100.0025 | Bachem | 25.0g | EUR 113 |
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Description: Sum Formula: C14H19NO4; CAS# [2018-66-8] |
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Z-Leu-OH (oil) |
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| C-2100.0100 | Bachem | 100.0g | EUR 171 |
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Description: Sum Formula: C14H19NO4; CAS# [2018-66-8] |
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) H-Thr-Ome(Oil) |
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| A6739-100000 | ApexBio | 100 g | EUR 316 |
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Description: H-Thr-Ome(Oil) |
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H-Thr-Ome(Oil) |
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Description: H-Thr-Ome(Oil) |
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 Vacuum pump, oil free |
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| FB-P-033A | FilterBio | 1set | EUR 432.75 |
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Description: Vacuum pump, oil free |
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 Cinnamon oil, from leaf |
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Cinnamon oil, from leaf |
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 Castor oil, EP grade |
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Castor oil, EP grade |
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| GL4383-500ML | Glentham Life Sciences | 500 ml | EUR 46 |
 Silicone oil, 500 cSt |
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| GK1916-1L | Glentham Life Sciences | 1 l | EUR 150 |
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Silicone oil, 500 cSt |
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Ethoxylated hydrogenated castor oil |
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| GK2628-200G | Glentham Life Sciences | 200 g | EUR 166 |
 Silicone oil, 350 cSt |
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| GK4111-100ML | Glentham Life Sciences | 100 ml | EUR 46 |
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Silicone oil, 350 cSt |
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| GK4111-1L | Glentham Life Sciences | 1 l | EUR 94 |
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Silicone oil, 350 cSt |
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 Oil Red O Solution |
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| ORG125 | ScyTek Laboratories | 125 ml | EUR 76 |
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Oil Red O Solution |
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| ORG500 | ScyTek Laboratories | 500 ml | EUR 140 |
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Oil Red O Solution |
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 Xylene - Peanut Oil Solution |
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| XPO125 | ScyTek Laboratories | 125 ml | EUR 72 |
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Xylene - Peanut Oil Solution |
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| XPO500 | ScyTek Laboratories | 500 ml | EUR 104 |
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Xylene - Peanut Oil Solution |
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 Fine natural hair brush for mounting sections |
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| PO501 | FD Neurotechnologies | each | EUR 65.53 |
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Description: Best quality products |
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| 2434-200 | Biovision | EUR 316 | |
 Capsaicin, natural |
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| GP1219-1G | Glentham Life Sciences | 1 g | EUR 91 |
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Capsaicin, natural |
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| GP1219-250MG | Glentham Life Sciences | 250 mg | EUR 59 |
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Capsaicin, natural |
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| GP1219-5G | Glentham Life Sciences | 5 g | EUR 245 |
 Resveratrol, natural |
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| GP2549-100MG | Glentham Life Sciences | 100 mg | EUR 62 |
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Resveratrol, natural |
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| GP2549-1G | Glentham Life Sciences | 1 g | EUR 166 |
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Resveratrol, natural |
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| GP2549-250MG | Glentham Life Sciences | 250 mg | EUR 94 |
) DiI in vegetable oil: (0.5mL) |
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| 60018 | Biotium | 0.5ML | EUR 204 |
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Description: Minimum order quantity: 1 unit of 0.5ML |
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 Neuro-dio in vegetable oil |
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| 60019 | Biotium | 0.2ML | EUR 259 |
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Description: Minimum order quantity: 1 unit of 0.2ML |
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 Neuro-DiI in vegetable oil |
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| 60020 | Biotium | 0.2ML | EUR 259 |
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Description: Minimum order quantity: 1 unit of 0.2ML |
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) Z-D-Leu-OH (oil) |
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| C-2105.0005 | Bachem | 5.0g | EUR 113 |
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Description: Sum Formula: C14H19NO4; CAS# [28862-79-5] |
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Z-D-Leu-OH (oil) |
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| C-2105.0025 | Bachem | 25.0g | EUR 335 |
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Description: Sum Formula: C14H19NO4; CAS# [28862-79-5] |
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Z-D-Leu-OH (oil) |
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| C-2105.0100 | Bachem | 100.0g | EUR 959 |
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Description: Sum Formula: C14H19NO4; CAS# [28862-79-5] |
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) Oil Red O (C.I. 26125) |
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| GT6537-100G | Glentham Life Sciences | 100 g | EUR 70 |
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Oil Red O (C.I. 26125) |
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| GT6537-25G | Glentham Life Sciences | 25 g | EUR 44 |
 100-1000ul Natural Tip, Natural, Sterile, 100/Rack |
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| BT222-RS | Bio Basic | 1PK, 100UNIT | EUR 54.94 |
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-Abscisic Acid (natural)) (+)-Abscisic Acid (natural) |
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| A153-100MG | TOKU-E | 100 mg | EUR 119 |
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(+)-Abscisic Acid (natural) |
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| A153-1G | TOKU-E | 1 g | EUR 366 |
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(+)-Abscisic Acid (natural) |
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| A153-250MG | TOKU-E | 250 mg | EUR 164 |
) Boc-N-Me-Nle-OH (oil) |
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| 5-05519 | CHI Scientific | 5g | Ask for price |
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Boc-N-Me-Nle-OH (oil) |
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| 5-05520 | CHI Scientific | 25g | Ask for price |
(isopropyl)-OH (oil)) Boc-Lys(Z)(isopropyl)-OH (oil) |
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| A-2890.0001 | Bachem | 1.0g | EUR 345 |
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Description: Sum Formula: C22H34N2O6; CAS# [125323-99-1] |
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Boc-Lys(Z)(isopropyl)-OH (oil) |
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| A-2890.0005 | Bachem | 5.0g | EUR 1301 |
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Description: Sum Formula: C22H34N2O6; CAS# [125323-99-1] |
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 Olive oil, extra virgin, EP grade |
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| GL3812-100ML | Glentham Life Sciences | 100 ml | EUR 45 |
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Olive oil, extra virgin, EP grade |
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| GL3812-1L | Glentham Life Sciences | 1 l | EUR 80 |
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Olive oil, extra virgin, EP grade |
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| GL3812-500ML | Glentham Life Sciences | 500 ml | EUR 59 |
 Paraffin oil, BP, Ph. Eur. grade |
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| GL4141-1L | Glentham Life Sciences | 1 l | EUR 58 |